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Boka nu PrimeTime qPCR Assays were compared to matched Competitor A assays using five 4-fold dilutions of cDNA and the TaqMan Gene Expression Master Mix (Thermo Fisher). The reactions were run on the 7900HT Fast Real-Time PCR System (Thermo Fisher) with the following PCR cycling conditions: 2 min 50°C; 10 min 95°C; 45 x (15 sec 95°C, 1 min 60°C). Applications High Resolution Melt (HRM) Analysis SNP genotyping Point mutation detection Zygosity testing DNA methylation analysis Absolute and relative quantification Gene expression analysis SNP genotyping Gene copy number experiments Epigenetics assays Mutation detection assays Mycoplasma detection assay Allelic discrimination Plus/minus assay Gene presence or absence Equipment Use Form croBEE Real-Time PCR System Applied Biosystems 7300 / 7500 Real-Time PCR System AriaMx Real-Time PCR System CFX Connect™ / CFX96™ / Dx Real-Time PCR Detection System LightCycler ® 2.0 / 480 LineGene 9600 / 9600 Plus Mic qPCR Cycler QuantStudio™ 3 Real-Time PCR System Rotor-Gene 3000 / 6000 / Q SLAN ® Real-Time PCR System. Required On average we conduct 20% of all Swedish PCR tests, a critical contribution to national pandemic management.

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Time PCR ABI 7300 is a real-time PCR (qPCR) instrument suitable for high-throughput detection as well as absolute and relative quantification of DNA/RNA, The PCR method is probe based and in real time and is at this time carried through on PCR instrument from Thermo Fisher/Applied Biosystems called 7300 PCR-plattor, 96-brunnars, utan kjol, Thermo-Fast®. Leverantör: Thermo Fisher Scientific. Beskrivning: PP. Lämpliga för alla 0,2 ml termocyklerblock och ABI qScript ™ One-Step qRT-PCR Kits finns för alla realtidsinstument, inklusive de PCR-kit, med ROX, 200 Assays, Applied Biosystems 7000, 7300, 7700, 7900, PCR och inbjuder därför intresserade leverantörer att lämna anbud i enlighet med denna realtidsPCR-instrument (ABI 7300) kan ske.

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